Recombinant production of TEV cleaved human parathyroid hormone
نویسندگان
چکیده
منابع مشابه
Recombinant human parathyroid hormone 1-84 (Natpara)
Description/Mechanism of Action Recombinant human parathyroid hormone (rhPTH 1-84) is identical to endogenous parathyroid hormone (PTH) and binds PTH-1 receptors in the bone, kidney, and has an indirect effect on calcium reabsorption in the intestine. It increases serum calcium by increasing renal tubular calcium reabsorption, intestinal calcium absorption, and bone turnover. Indication under R...
متن کاملPurification and characterization of recombinant human parathyroid hormone-related protein.
Full-length human parathyroid hormone-related protein (PTHrP-(1-141] as well as a carboxyl-terminal shortened form (PTHrP-(1-108] have been expressed from recombinant DNA-derived clones. These proteins were expressed in Escherichia coli as fusion proteins so that cyanogen bromide cleavage yields the desired product. Both proteins were purified and then characterized by sodium dodecyl sulfate ge...
متن کاملUse of recombinant human parathyroid hormone in hypocalcemic cardiomyopathy.
Hypocalcemia secondary to hypoparathyroidism is a rare cause of congestive heart failure. However, its early recognition and treatment lead to significant improvement in cardiac function. We report a middle-aged woman presenting with symptoms of heart failure with a serum calcium level of 3.7 mg/dl and a serum inorganic phosphate level of 17.6 mg/dl 22 years after subtotal thyroidectomy. Beside...
متن کاملEvaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in Pichia Pastoris
Recombinant protein production in Pichia pastoris is based on alcohol oxidase promoterswhich are regulated by methanol. However, the use of methanol has several disadvantages,which is why current trends in bioprocess development with Pichia pastoris (P. pastoris) arefocusing on methanol mixed feeding strategies. This work aimed to develop a new experimentalmethod and compare the effect of vario...
متن کاملEvaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in Pichia Pastoris
Recombinant protein production in Pichia pastoris is based on alcohol oxidase promoterswhich are regulated by methanol. However, the use of methanol has several disadvantages,which is why current trends in bioprocess development with Pichia pastoris (P. pastoris) arefocusing on methanol mixed feeding strategies. This work aimed to develop a new experimentalmethod and compare the effect of vario...
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ژورنال
عنوان ژورنال: Journal of Peptide Science
سال: 2013
ISSN: 1075-2617
DOI: 10.1002/psc.2528